SAT test
The test offers genetic information invisible under the microscope, which can be key to choosing the right strategy and achieving a successful pregnancy.
SAT (Sperm Aneuploidy Test) is a modern molecular test that analyzes the chromosomal profile of sperm in order to detect the presence of aneuploidies — chromosomal abnormalities that can be the cause of male infertility, unsuccessful IVF attempts and recurrent miscarriages.
What is aneuploidy?
Aneuploidy means the presence of an abnormal number of chromosomes in the cell — for example, one in more or less than the normal pair. In sperm, this may mean that instead of 23 chromosomes, they carry 22 or 24, which increases the risk of abnormal embryonic development or failed implantation.
What does the SAT study?
Using FISH (fluorescence in situ hybridization) technology, SAT examines thousands of sperm simultaneously for the presence of aneuploidies in the most commonly affected chromosomes:
- Sex chromosomes: X, Y;
- Chromosomes 13, 18, 21 — associated with syndromes such as Down, Edwards, Patau.
When is the SAT test recommended?
SAT is especially indicated in:
- Men with normal indicators, but unexplained infertility;
- Repeated miscarriages;
- Unsuccessful in vitro attempts with good embryos;
- Severe deviations in the spermogram — oligozoospermia, asthenozoospermia, teratozoospermia;
- Age over 40 years.
How is the test performed?
1. Collection of semen sample — after 2—5 days of sexual abstinence
2. Laboratory processing and fixation of spermatozoa;
3. Hybridization with fluorescent probes — which bind to specific chromosomes;
4. Analysis under a fluorescent microscope — count at least 5,000 sperm.
How are the results interpreted?
The results showed a percentage of sperm with aneuploidies on each chromosome examined. An increased frequency can point to:
- Increased risk of chromosomal abnormalities in embryos;
- Need for pre-implantation genetic diagnosis (PGT-A);
- Recommendation for the selection of donor sperm in severe deviations.
What do we offer in our clinic?
For the first time in Bulgaria, the genetic test for xpomosome anomalies in the clinic of the Invitro Clinic “Sofia”. We offer:
- Consultation in the interpretation of the results;
- Individual approach in making therapeutic decisions;
- Possibility of additional tests (Halosperm, CatSper, PGT-A) if necessary.
Conclusion
The SAT is a valuable tool for clarifying the causes of male infertility and failures in assisted reproduction. The test offers genetic information invisible under the microscope, which can be key to choosing the right strategy and achieving a successful pregnancy.