HBA test
HBA (Hyaluronan Binding Assay) is a diagnostic test that measures the ability of sperm to bind to hyaluronan, a natural component of the shell around the egg.
HBA test — assessment of sperm maturity and functionality
HBA (Hyaluronan Binding Assay) is a diagnostic test that measures the ability of sperm to bind to hyaluronan, a natural component of the shell around the egg. This binding is characteristic only of mature and functionally fit spermatozoa, which have the greatest chance of fertilizing the egg.
What is hyaluronan?
Hyaluronan (hyaluronic acid) is a natural polysaccharide found in the outer shell of the ovum (cumulus oophorus). During the final stage of their maturation, sperm acquire the ability to bind to hyaluronan. A number of studies have shown that the ability to recognize and attach to hyaluronan is an important indicator for proving the maturity and fertilization capacity of male gametes. Only mature sperm that have undergone complete spermatogenesis have receptors for binding to hyaluronan - a natural biological selection mechanism.
What does the HBA test show?
The HBA estimates the percentage of sperm that can bind to hyaluronan. This is an indirect indicator of:
- Physiological maturity;
- DNA integrity;
- Normal morphology;
- Low risk of chromosomal abnormalities.
How is the test carried out?
- Collection of a sperm sample after standard abstinence (2—5 days);
- Placing on a special surface with hyaluronan;
- After a certain time — microscopic counting of connected and unrelated spermatozoa;
- Calculation of connection percentage (HBA index).
How are the results interpreted?
The results are presented as the percentage of sperm bound and have important clinical significance when choosing a method of fertilization.
- Low HBA score often correlates with high DNA fragmentation of sperm;
- May be an indirect marker of lower embryonic quality;
- It does not replace the Halosperm test, but complements the assessment of the functional state of sperm.
When is the HBA test recommended?
- Unexplained male infertility;
- Normal spermogram, but unsuccessful attempts to conceive;
- Increased DNA fragmentation;
- Previous unsuccessful IVF cycles;
- Choice between IVF and ICSI procedures.
What do we offer in our clinic?
In Vitro Clinic “Sofia” the HBA test is part of the extended evaluation of male fertility. Advantages:
- Fast and reliable research;
- Using the result to select a method of fertilization;
- Possibility of additional sperm selection.
Conclusion
The HBA test provides important functional information about sperm quality that is not visible on a standard spermogram. It supports the individualization of treatment and increases the chance of successful fertilization and a healthy pregnancy.